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Mirror slides for high-sensitivity cell and tissue fluorescence imaging

Abstract : Fluorescence microscopy has become the method of choice in the majority of life-science applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with wide field of observation and large depth of focus, two major breakthroughs for routine analysis and high-throughput screening applications on cells and tissue samples.
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https://hal.archives-ouvertes.fr/hal-02518119
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Eric Le Moal, Emmanuel Fort, Sandrine Lévêque-Fort, Anne Janin, Hideyuki Murata, et al.. Mirror slides for high-sensitivity cell and tissue fluorescence imaging. Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2007, 12 (2), pp.024030. ⟨10.1117/1.2722730⟩. ⟨hal-02518119⟩

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