Evidence for ACD5 ceramide kinase activity involvement in Arabidopsis response to cold stress

Abstract : Although sphingolipids emerged as important signals for plant response to low temperature, investigations have been limited so far to the function of long-chain base intermediates. The formation and function of ceramide phosphates (Cer-Ps) in chilled Arabidopsis were explored. Cer-Ps were analysed by thin layer chromatography (TLC) following in vivo metabolic radiolabelling. Ceramide kinase activity, gene expression and growth phenotype were determined in unstressed and cold-stressed wild type (WT) and Arabidopsis ceramide kinase mutant acd5. A rapid and transient formation of Cer-P occurs in cold-stressed WT Arabidopsis plantlets and cultured cells, which is strongly impaired in acd5 mutant. Although concomitant, Cer-P formation is independent of long-chain base phosphate (LCB-P) formation. No variation of ceramide kinase activity was measured in vitro in WT plantlets upon cold stress but the activity in acd5 mutant was further reduced by cold stress. At the seedling stage, acd5 response to cold was similar to that of WT. Nevertheless, acd5 seed germination was hypersensitive to cold and abscisic acid (ABA), and ABA-dependent gene expression was modified in acd5 seeds when germinated at low temperature. Our data involve for the first time Cer-P and ACD5 in low temperature response and further underline the complexity of sphingolipid signalling operating during cold stress.
Document type :
Journal articles
Complete list of metadatas

Cited literature [8 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-01229102
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Monday, November 16, 2015 - 12:42:34 PM
Last modification on : Friday, June 28, 2019 - 11:00:03 AM
Long-term archiving on : Friday, April 28, 2017 - 9:51:01 PM

File

Dutilleul_2015_Evidence_for_AC...
Files produced by the author(s)

Identifiers

Citation

Christelle Dutilleul, Heidy Chavarria, Nathalie Rézé, Bruno Sotta, Emmanuel Baudouin, et al.. Evidence for ACD5 ceramide kinase activity involvement in Arabidopsis response to cold stress. Plant, Cell and Environment, Wiley, 2015, 38 (12), pp.2688-2697 ⟨10.1111/pce.12578⟩. ⟨hal-01229102⟩

Share

Metrics

Record views

236

Files downloads

277